Workshop | 14 September, 10h00-13h00 | Direct Digital Manufacturing: nano-manufacturing
10h00 – Open Session |Nuno Alves, Diretor CDRSP-IPLeiria
10h15 – From micro to nano: a pathway to tailor biomedical applications | Pedro Morouço, CDRSP-IPLeiria
10h45 – Mimicking the double scale wrinkles of 2D surfaces for 3D printing | Paula Piedade, CEMMPRE, DEM, FCTUC
11h30 – Coffee Break
11h45 – Two-photon polymerization based 3D microprinting for Bio-medical applications | Rameez Ahmad, Nanoscribe GmbH
13h00 – Lunch
FROM MICRO TO NANO : A PATHWAY TO TAILOR BIOMEDICAL APPLICATIONS
Only the complexity of human tissues could justify that in the 80’s Tissue Engineering (TE) emerged as a scientific field with an enormous potential. Targeting to regenerate bone, cartilage, skin, or other tissues and organs, bridging the anatomy with its physiology/function is a paramount challenge to be solved. Several efforts have been made, by research groups spread worldwide, to develop constructs that could mimic native tissues. Due to its nature TE gathers scientists, engineers and physicians in multidisciplinary teams using a variety of methods to construct biological substitutes. While there are different approaches used for TE, the most emergent and promising method is biofabrication. We have recently defined it as “the automated generation of biologically functional products with structural organization from living cells, bioactive molecules, biomaterials, cell aggregates such as micro-tissues, or hybrid cell-material constructs, through Bioprinting or Bioassembly and subsequent tissue maturation processes”.
Bioprinting is attracting a lot of research teams presenting an exponential increase in published works for the last 3 years. Meanwhile, 3D bioprinting market was valued at $98.6 million in 2015, and an annual grow of 36% for the next 6 years is expected. The existing and expected progress on printing technology will be presented.
Pedro Morouço, CDRSP-IPLeiria
MIMICKING THE DOUBLE SCALE WRINKLES OF 2D SURFACES FOR 3 D PRINTING
The 3D printing of scaffolds of polymeric materials has reached its cruise phase. Nevertheless, in order to really explore all the features of polymeric additive manufacturing some challenges still remain unsolved, namely the ability to produce the desired 3D architecture but with the appropriate topographic surface. This challenge is upraised when considering that most of the developed work indicates that sub-micron and nanometric topographies are most relevant for the interaction of the produced biomaterial with the eukaryotic cells. In this presentation it is exemplified the production of double scale wrinkled surfaces, by sputtering, with different chemistry obtained by using graphite and polyamide targets. The influence of chemical and topographic cues was evaluated by studying their interface with rat embryo cortex cells.
Paula Piedade, CEMMPRE, DEM, FCTUC
TWO-PHOTON POLYMERIZATION BASED 3D MICROPRINTING FOR BIO-MEDICAL APPLICATIONS
The achievements of the two-photon polymerization based world’s highest resolution 3D micro-printer will be presented. The device offers a defined control on the feature sizes and resolutions ranging from nanometer to micrometer scale and accessible print area of ~ cm2. The complete freedom of design in three dimensions that our device offers, when coupled with intuitive minds of our customers, opens new frontiers in the field of metamaterials, bio-medical, micro robotics, microfluidics etc.
In this talk, this art of 3D micro-printing will be explained with a focus on customers’ applications in the field of bio-medical and life sciences. Prominent examples include artificially designed and μ-CT scan derived 3D cell scaffolds, magnetically actuated microrobots for targeted drug delivery and for transport of individual sperm cells etc.
Rameez Ahmad, Nanoscribe GmbH